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MALACHITE GREEN FOR MICROSCOPY
MALACHITE GREEN FOR MICROSCOPY
Malachite green has classically been used by the light microscopist for the demonstration of fungi and bacterial spores. It is a weakly basic diaminotri- phenylmethane dye with useful indicator properties at both very high and very low pH Malachite Green is used for bacterial spore staining by Schaeffer and Fulton`s method. It can also be used in place of methyl green in the Pappenheim stain, when combined with the Gram stain.
It is used as a biological stain for microscopic analysis of cell biology and tissue samples. In the Gimenez staining method, basic fuchsin stains bacteria red or magenta, and malachite green is used as a blue-green counterstain. ... Malachite green is in a class of molecules called fluorophores.
Typically cures ich in 24 hours, treatment can be repeated in 24-48 hours, if necessary. If fish experience stress, change 50% of the water. Sometimes treatment will take 10 days to fully irradiate infestations.
Malachite green is used for routine staining methods in bacteriology, e. g. the spore-staining method acc. to Rakette (differential spore staining with malachite green in bacteriological samples). The dye is used in a hot staining method to stain specifically target struc tures. Counterstain with a red dye.