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A chemical substance, also known as a pure substance, is a form of matter that has a constant chemical composition and characteristic properties. It cannot be separated into components by physical separation methods, i.e., without breaking chemical bonds Chemical substances can be chemical elements, chemical compounds, ions or alloys.

Chemical substances are often called 'pure' to set them apart from mixtures. A common example of a chemical substance is pure water; it has the same properties and the same ratio of hydrogen to oxygen whether it is isolated from a river or made in a laboratory. Other chemical substances commonly encountered in pure form are diamond (carbon), gold, table salt (sodium chloride) and refined sugar (sucrose). However, in practice, no substance is entirely pure, and chemical purity is specified according to the intended use of the chemical.

Chemical substances exist as solids, liquids, gases, or plasma, and may change between these phases of matter with changes in temperature or pressure. Chemical substances may be combined or converted to others by means of chemical reactions.
Forms of energy, such as light and heat, do not matter and are thus not "substances" in this regard.

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