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Lab Chemical-60
DIACETYL FLUORESCEIN
SKU: RXSOL-60-6604-420
Fluorescein diacetate (FDA) is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product.
Application
To prepare a FDA stock solution 5 mg of fluorescein diacetate is dissolved in 1 ml acetone.
2,5 ml cell culture medium is cooled on ice.
50 ml of the FDA stock solution is added and this diluted FDA solution is stored on ice.