It is employed as a dough conditioner in bread products. In biochemistry it is used for purification of proteins via ammonium sulfate precipitation method. Ammonium sulfate is used in agriculture as fertilizer. It is a slow releasing nitrogenous fertilizer and a rich source of sulphur nutrient. It finds use as raw materials for various chemical products such as dyes and pharmacetical products.

It is a component of flame-proofing agents and fire extinguisher powder.