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Pine oil is an essential oil obtained by the steam distillation of stumps, needles, twigs and cones from a variety of species of pine, particularly Pinus sylvestris. As of 1995, synthetic pine oil was the "biggest single turpentine derivative." Synthetic pine oils accounted for 90% of sales as of 2000.

In alternative medicine, it is said to be used in aromatherapy, as a scent in bath oils or more commonly as a cleaning product, and as a lubricant in small and expensive clockwork instruments. It may also be used varyingly as a disinfectant, sanitizer, mircobicide / microbistat, virucide, insecticide, and a massage oil.[citation needed] It is also used as an effective herbicide where its action is to modify the waxy cuticle of plants, resulting in desiccation.

Pine oil is distinguished from other products from pine, such as turpentine, the low-boiling fraction from the distillation of pine sap, and rosin, the thick tar remaining after turpentine is distilled.

Chemically, pine oil consists mainly of α-terpineo and other cyclic terpene alcohols. It may also contain terpene hydrocarbons, ethers, and esters. The exact composition depends on various factors, such as the variety of pine from which it is produced and the parts of the tree used.

ndustrially, pine oil is used as a frother in mineral extraction from ores. For example, in copper extraction pine oil is used to condition copper sulfide ores for froth flotation. Therefore, it is important in the industry for the froth flotation process. It has largely been replaced by synthetic alcohols and polyglycol ethers.